Recombinant WNK3 protein (His tag)

• DataSheet
• COA (Batch NP456610)
• Material Safety Data Sheets (MSDS)

Recombinant Human Serine/threonine-protein kinase WNK3 (EC 2.7.11.1) Fragment (aa 135-495) is a high-purity protein encompassing the complete protein kinase domain (aa 147-405). This fragment serves as an essential tool for investigating kinase activity, regulatory mechanisms in electrolyte homeostasis, and cellular responses to osmotic stress, enabling focused functional and structural studies.

Serine/threonine-protein kinase WNK3 is a key component of the WNK3-SPAK/OSR1 kinase cascade, crucial for regulating electrolyte balance and cellular volume under hyperosmotic stress. It functions as a molecular crowding sensor by undergoing liquid-liquid phase separation to form membraneless compartments, concentrating and activating downstream kinases OXSR1/OSR1 and STK39/SPAK. These kinases modulate ion cotransporters (e.g., SLC12A family members), promoting ion influx via Na-K-Cl cotransporters and inhibiting efflux via K-Cl cotransporters. WNK3 also acts as a scaffold, influencing membrane localization of transporters and channels like KCNJ1 and SLC26A9, and enhances Ca²⁺ influx through TRPV5/TRPV6. Predominantly cytoplasmic, it localizes to stress-induced membraneless compartments. This fragment retains the kinase domain, enabling targeted research into phosphorylation-dependent signaling and osmotic stress responses.

MKAVATSPSG RFLKFDIELG RGAFKTVYKG LDTETWVEVA WCELQDRKLT KAEQQRFKEE AEMLKGLQHP NIVRFYDSWE
SILKGKKCIV LVTELMTSGT LKTYLKRFKV MKPKVLRSWC RQILKGLQFL HTRTPPIIHR DLKCDNIFIT GPTGSVKIGD
LGLATLMRTS FAKSVIGTPE FMAPEMYEEH YDESVDVYAF GMCMLEMATS EYPYSECQNA AQIYRKVTSG IKPASFNKVT
DPEVKEIIEG CIRQNKSERL SIRDLLNHAF FAEDTGLRVE LAEEDDCSNS SLALRLWVED PKKLKGKHKD NEAIEFSFNL
ETDTPEEVAY EMVKSGFFHE SDSKAVAKSI RDRVTPIKKT RLEHHHHHH