-
Product Name
WDR35 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to WDR35
-
Tested applications
WB, IF
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
WDR35 antibody; CED2 antibody; IFT121 antibody; IFTA1 antibody; SRTD7 antibody; WD repeat domain 35 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 871-1170 of human WDR35 (NP_065830.2).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:1000
IF 1:50 - 1:200 -
Validations
Western blot - WDR35 Polyclonal Antibody
Western blot analysis of extracts of LO2 cells, using WDR35 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
-
Background
As a component of the IFT complex A (IFT-A), a complex required for retrograde ciliary transport, it is involved in ciliogenesis. May promote CASP3 activation and TNF-stimulated apoptosis.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"