Recombinant VPS25 protein (His tag)

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Recombinant human Vacuolar protein-sorting-associated protein 25 (hVps25) fragment (residues 1-176) is a high-quality protein designed for structural and biochemical studies. This fragment represents a stable, soluble unit of the ESCRT-II complex, essential for endosomal sorting and multivesicular body formation, offering researchers a reliable tool for investigating intracellular trafficking mechanisms.

Vacuolar protein-sorting-associated protein 25 (hVps25) is a critical component of the ESCRT-II complex, which mediates endosomal sorting and multivesicular body (MVB) formation. This process facilitates the delivery of transmembrane proteins to lysosomes for degradation and plays roles in transcription regulation and viral budding. The full-length protein localizes to the cytoplasm, endosome membranes, and nucleoplasm, exhibiting a diffuse cytoplasmic and nuclear distribution with punctate patterns when coexpressed with CHMP6. The recombinant fragment (residues 1-176) encompasses the functional domain, enabling focused studies on its mechanistic contributions to cellular transport pathways.