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Product Name
VPS16 antibody
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Description
VPS16 Rabbit Polyclonal antibody. Positive IHC detected in human liver tissue. Positive WB detected in HepG2 cells, HeLa cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 95kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
hVPS16 antibody; VPS16 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of VPS16 recombinant protein (Accession Number: BC073959). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:116773(VPS16 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human liver using Catalog No:116773(VPS16 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:116773(VPS16 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-VPS16 (IP:Catalog No:116773, 3ug; Detection:Catalog No:116773 1:500) with HeLa cells lysate 2480ug.
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Background
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. In yeast, Vps proteins are involved in the trafficking of endocytic and biosynthetic proteins to the vacuole, which functionally resembles the lysosome of higher organisms. Human VPS16 is the homolog of yeast class C Vps16 protein. VPS16 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.
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References
- Ganley IG, Wong PM, Gammoh N, Jiang X. Distinct autophagosomal-lysosomal fusion mechanism revealed by thapsigargin-induced autophagy arrest. Molecular cell. 42(6):731-43. 2011.
- Ulbricht A, Eppler FJ, Tapia VE. Cellular mechanotransduction relies on tension-induced and chaperone-assisted autophagy. Current biology : CB. 23(5):430-5. 2013.
- van der Kant R, Jonker CT, Wijdeven RH. Characterization of the Mammalian CORVET and HOPS Complexes and Their Modular Restructuring for Endosome Specificity. The Journal of biological chemistry. 290(51):30280-90. 2015.
- van der Kant R, Fish A, Janssen L. Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L. Journal of cell science. 126(Pt 15):3462-74. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"