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Product Name
VILIP 3 antibody
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Description
VILIP 3 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human gliomas tissue, human kidney tissue. Positive IP detected in mouse brain tissue. Positive WB detected in mouse brain tissue, HeLa cells. Observed molecular weight by Western-blot: 20-22 kDa
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Tested applications
ELISA, IHC, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BDR1 antibody; Calcium binding protein BDR 1 antibody; hippOCalcin like 1 antibody; HippOCalcin like protein 1 antibody; HLP2 antibody; HPCA antibody; HPCAL1 antibody; VILIP 3 antibody; Visinin like protein 3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of VILIP 3 recombinant protein (Accession Number: NM_001258358). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:116750(HPCA antibody) at dilution of 1:1000
IP Result of anti-HPCA (IP:Catalog No:116750, 3ug; Detection:Catalog No:116750 1:1000) with mouse brain tissue lysate 4000ug.
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:116750(HPCA antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:116750(HPCA Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
HPCAL1 represents hippocalcin-like 1
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References
- Zhang Y, Liu Y, Duan J. Hippocalcin-like 1 suppresses hepatocellular carcinoma progression by promoting p21(Waf/Cip1) stabilization by activating the ERK1/2-MAPK pathway. Hepatology (Baltimore, Md.). 63(3):880-97. 2016.
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