-
Product Name
VHL Monoclonal Antibody
- Documents
-
Description
Monoclonal antibody to VHL
-
Tested applications
WB, IHC, IF, IP
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
VHL antibody; HRCA1 antibody; RCA1 antibody; VHL1 antibody; pVHL antibody; von Hippel-Lindau tumor suppressor antibody
-
Isotype
Mouse IgG
-
Preparation
Antigen: Recombinant protein of human VHL
-
Clonality
Monoclonal
-
Formulation
PBS with 0.02% sodium azide, pH7.3.
-
Storage instructions
Store at 4℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
IP 1:20 - 1:50 -
Validations
Western blot - VHL Monoclonal Antibody
Western blot analysis of extracts of various cell lines, using VHL antibody .Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
-
Background
Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. Ubiquitinates, in an oxygen-responsive manner, ADRB2.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"