Vasoactive intestinal peptide, VIP

Vasoactive intestinal peptide, VIP

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

HSDAVFTDNYSRFRKQMAVKKYLNSVLT

H-His-Ser-Asp-Ala-Val-Phe-Thr-Asp-Asn-Tyr-Ser-Arg-Phe-Arg-Lys-Gln-Met-Ala-Val-Lys-Lys-Tyr-Leu-Asn-Ser-Val-Leu-Thr-NH2

28

97.92%

C₁₄₈H₂₃₃N₄₃O₄₂S

3318.7

Synthetic

Powder

Vasoactive intestinal peptide (VIP) is a 28-amino acid neuropeptide originally isolated from the intestine. In avian species, such as the chicken, VIP differs from its mammalian counterpart by four amino acids. This peptide is synthesized in hypothalamic neurons, particularly within the medial basal hypothalamus, and is transported to nerve terminals in the median eminence. Immunohistochemical studies demonstrate that VIP-containing neurons and their terminals are more abundant and exhibit increased immunoreactivity in incubating birds compared to laying birds. Radioimmunoassay data further indicate significantly higher concentrations of VIP in the median eminence and medial basal hypothalamus during incubation. These observations suggest that hypothalamic VIP functions as a physiologically significant prolactin-releasing factor in avian neuroendocrine regulation.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Nilsson A. Structure of the vasoactive intestinal octacosapeptide from chicken intestine. The amino acid sequence. FEBS Lett. 1975;60(2):322-6.
• Sharp PJ, Sterling RJ, Talbot RT, Huskisson NS. The role of hypothalamic vasoactive intestinal polypeptide in the maintenance of prolactin secretion in incubating bantam hens: observations using passive immunization, radioimmunoassay and immunohistochemistry. J Endocrinol. 1989 Jul;122(1):5-13. doi: 10.1677/joe.0.1220005. PMID: 2769162.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.