VAPB antibody

Cat.#: 116715

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Product Information

  • Product Name
    VAPB antibody
  • Documents
  • Description
    VAPB Rabbit Polyclonal antibody. Positive IHC detected in human pancreas cancer tissue, human breast cancer tissue. Positive IF detected in HepG2 cells. Positive FC detected in HepG2 cells. Positive IP detected in HeLa cells. Positive WB detected in Jurkat cells, HeLa cells, HepG2 cells, human brain tissue, mouse brain tissue, mouse liver tissue, mouse skeletal muscle tissue. Observed molecular weight by Western-blot: 27 kDa
  • Tested applications
    ELISA, WB, IHC, IF, IP, FC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    ALS8 antibody; VAMP assOCiated protein B/C antibody; VAMP B antibody; VAMP B/VAMP C antibody; VAMP C antibody; VAP B antibody; VAP B/VAP C antibody; VAP C antibody; VAPB antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of VAPB recombinant protein (Accession Number: NM_004738). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    Immunofluorescent analysis of HepG2 cells, using VAPB antibody Catalog No:116715 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    Immunofluorescent analysis of HepG2 cells, using VAPB antibody Catalog No:116715 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    IP Result of anti-VAPB (IP:Catalog No:116715, 3ug; Detection:Catalog No:116715 1:800) with HeLa cells lysate 1840ug.

    IP Result of anti-VAPB (IP:Catalog No:116715, 3ug; Detection:Catalog No:116715 1:800) with HeLa cells lysate 1840ug.

    1X10^6 HepG2 cells were stained with 0.2ug VAPB antibody (Catalog No:116715, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 HepG2 cells were stained with 0.2ug VAPB antibody (Catalog No:116715, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:116715(VAPB antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:116715(VAPB antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:116715(VAPB antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:116715(VAPB antibody) at dilution of 1:100 (under 40x lens)

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:116715(VAPB antibody) at dilution of 1:500

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:116715(VAPB antibody) at dilution of 1:500

  • Background
    Vesicle-associated membrane protein-associated protein B (VAPB) is an integral membrane protein localized to the endoplasmic reticulum (ER) membrane. VAPB has been implicated in various cellular processes, including ER stress, the unfolded protein response (UPR) and calcium homeostasis regulation. The mutations in the gene of VAPB cause amyotrophic lateral sclerosis 8 (ALS8) and some other related forms of motor neuron disease including late onset spinal muscular atrophy.
  • References
    • McCray BA, Skordalakes E, Taylor JP. Disease mutations in Rab7 result in unregulated nucleotide exchange and inappropriate activation. Human molecular genetics. 19(6):1033-47. 2010.
    • Qiu L, Qiao T, Beers M. Widespread aggregation of mutant VAPB associated with ALS does not cause motor neuron degeneration or modulate mutant SOD1 aggregation and toxicity in mice. Molecular neurodegeneration. 8:1. 2013.
    • Deidda I, Galizzi G, Passantino R. Expression of vesicle-associated membrane-protein-associated protein B cleavage products in peripheral blood leukocytes and cerebrospinal fluid of patients with sporadic amyotrophic lateral sclerosis. European journal of neurology. 21(3):478-85. 2014.
    • Shima K, Klinger M, Schütze S. The role of endoplasmic reticulum-related BiP/GRP78 in interferon gamma-induced persistent Chlamydia pneumoniae infection. Cellular microbiology. 17(7):923-34. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"