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  • USP33 antibody
  • USP33 antibody
  • USP33 antibody
  • USP33 antibody

USP33 antibody

Cat.#: 116607

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Special Price 239.0 USD

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Product Information

  • Product Name
    USP33 antibody
  • Documents
  • Description
    USP33 Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells, mouse brain tissue. Positive IHC detected in human prostate cancer tissue, human kidney tissue. Observed molecular weight by Western-blot: 103 kDa,107 kDa
  • Tested applications
    ELISA, WB, IHC, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    Deubiquitinating enzyme 33 antibody; hVDU1 antibody; KIAA1097 antibody; Ubiquitin thiolesterase 33 antibody; USP33 antibody; VDU1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of USP33 recombinant protein (Accession Number: BC016663). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:1000

    IHC: 1:20-1:200

  • Validations

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:116607(USP33 antibody) at dilution of 1:300

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:116607(USP33 antibody) at dilution of 1:300

    Immunohistochemistry of paraffin-embedded human prostate cancer slide using Catalog No:116607(USP33 Antibody) at dilution of 1:50(under 10x lens)

    Immunohistochemistry of paraffin-embedded human prostate cancer slide using Catalog No:116607(USP33 Antibody) at dilution of 1:50(under 10x lens)

    Immunohistochemistry of paraffin-embedded human prostate cancer slide using Catalog No:116607(USP33 Antibody) at dilution of 1:50(under 40x lens)

    Immunohistochemistry of paraffin-embedded human prostate cancer slide using Catalog No:116607(USP33 Antibody) at dilution of 1:50(under 40x lens)

    IP Result of anti-USP33 (IP:Catalog No:116607, 4ug; Detection:Catalog No:116607 1:300) with HEK-293 cells lysate 2400ug.

    IP Result of anti-USP33 (IP:Catalog No:116607, 4ug; Detection:Catalog No:116607 1:300) with HEK-293 cells lysate 2400ug.

  • Background
    USP33(Ubiquitin carboxyl-terminal hydrolase 33) is also named as KIAA1097, VDU1 and belongs to the peptidase C19 family. This protein, originally identified as a von Hippel-Lindau tumor suppressor (VHL) protein-interacting deubiquitinating enzyme, binds to the Robo1 receptor, and that USP33 is required for Slit responsiveness in breast cancer cells(PMID:19706539). The localization of USP33 is broadly confined to the secretory pathway, with all variants localizing to endoplasmic reticulum-associated structures(PMID:21801292). It has 3 isoforms produced by alternative splicing. This antibody is specific to USP33.
  • References
    • Wen P, Kong R, Liu J. USP33, a new player in lung cancer, mediates Slit-Robo signaling. Protein & cell. 5(9):704-13. 2014.
  • Antibodies
  • cDNA Clones

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"