UBE2S Rabbit Polyclonal antibody. Positive IP detected in A2780 cells. Positive WB detected in MDA-MB-453s cells, A2780 cells, human liver tissue, MCF7 cells, mouse liver tissue, mouse ovary tissue. Positive FC detected in Jurkat cells. Positive IHC detected in human breast cancer tissue, human ovary tumor tissue, human skin tissue. Observed molecular weight by Western-blot: 28 kDa
ELISA, WB, IHC, FC, IP
Human,Mouse,Rat; other species not tested.
E2 EPF antibody; E2EPF antibody; EPF5 antibody; UBE2S antibody; Ubiquitin carrier protein S antibody; Ubiquitin protein ligase S antibody
This antibody was obtained by immunization of UBE2S recombinant protein (Accession Number: BC066948). Purification method: Antigen affinity purified.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20℃. DO NOT ALIQUOT
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with Catalog No:116535(UBE2S antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:116535(UBE2S antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:116535(UBE2S antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-UBE2S (IP:Catalog No:116535, 4ug; Detection:Catalog No:116535 1:1000) with A2780 cells lysate 960ug.
1X10^6 Jurkat cells were stained with 0.5ug UBE2S antibody (Catalog No:116535, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
UBE2S(Ubiquitin-conjugating enzyme E2 S) is also named as E2EPF and belongs to the ubiquitin-conjugating enzyme family. It acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C) and a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Overexpression of UBE2S can increase tumor cell proliferation, invasion, and metastasis through the VHL-HIF pathway (PMID:16819549).
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