UBC12 antibody

Cat.#: 116476

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Product Information

  • Product Name
    UBC12 antibody
  • Documents
  • Description
    UBC12 Rabbit Polyclonal antibody. Positive IF detected in L02 cells. Positive WB detected in L02 cells, SMMC-7721 cells. Positive IP detected in L02 cells. Observed molecular weight by Western-blot: 21 kDa
  • Tested applications
    ELISA, WB, IF, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    hUbc12 antibody; NEDD8 carrier protein antibody; NEDD8 conjugating enzyme Ubc12 antibody; NEDD8 protein ligase antibody; UBC RS2 antibody; UBC12 antibody; UBE2M antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of UBC12 recombinant protein (Accession Number: NM_003969). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:2000

    IF: 1:20-1:200

  • Validations

    L02 cells were subjected to SDS PAGE followed by western blot with Catalog No:116476(UBC12 Antibody) at dilution of 1:600

    L02 cells were subjected to SDS PAGE followed by western blot with Catalog No:116476(UBC12 Antibody) at dilution of 1:600

    IP Result of anti-UBC12 (IP:Catalog No:116476, 4ug; Detection:Catalog No:116476 1:600) with L02 cells lysate 1800ug.

    IP Result of anti-UBC12 (IP:Catalog No:116476, 4ug; Detection:Catalog No:116476 1:600) with L02 cells lysate 1800ug.

    Immunofluorescent analysis of L02 cells using Catalog No:116476(UBC12 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of L02 cells using Catalog No:116476(UBC12 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    UBE2M represents ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"