Three letter code
Peptide Purity (HPLC)
Proline-rich antimicrobial peptides (PrAMPs) are antibacterial components of the immune system of some animals. Mardirossian et al. identified two PrAMPs in the artiodactyl Tursiops truncatus (bottlenose dolphin), Tur1A and Tur1B. Tur1A was shown to inhibit bacterial protein synthesis by binding to the ribosome. The proline-rich AMPs Tur1A and Tur1B were identified in the bottlenose dolphin. Tur1A is non-lytic and inhibits bacterial protein synthesis. Tur1A binds within the ribosomal tunnel and prevents translation elongation. Tur1B is a poor inhibitor of translation and thus probably has another target. Peptide truncation indicates that the antimicorbial activity is mainly located at the N-terminal region residues 1-16 (MIC 4 uM). While the intact molecule shows similar activity against sbmA and yjiL KO strains, the truncated peptides 1-16, 8-24, and 16-32 are inactive, indicating the entire sequence do not depend on the Pro-rich peptide transporters for activity. Tur1A inhibits E. coli protein translation by binding to the exit tunnel of ribosome in a reversed order. Interestingly, residues 1-16 plays a major role in ribosome binding.
Ideally Tur1A peptide should be stored in a freezer at or below -9C. Tur1A peptide should be refrigerated after reconstitution. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides
- Cell Chem Biol. 2018 Feb 22. in press. doi: 10.1016/j.chembiol.2018.02.004.
About TFA salt
Trifluoroacetic acid (TFA) is a strong acid, which is commonly used to cleave synthesized peptides from solid-phase resins and is also used to improve HPLC performance in the peptide purification step. By default, custom peptides are delivered as lyophilized TFA salts, and can contain as much as 10-45% TFA.
TFA in custom peptides can cause inexplicable discrepancies in subsequent assay data. For instance, TFA in nM concentrations has been shown to interfere with cellular assays, inhibiting cellular proliferation in some instances, and increasing cell viability in others . It has also been found to be an unintended allosteric modulator of the glycine receptor, GlyR.
TFA Removal Service is recommended for:
- Peptides that will be used in cellular assays
- Peptides that will be used as APIs or in manufactured products
- For hydrophilic peptides containing numerous basic residues
- Analysed Sequence:H-RRIRFRPPYLPRPGRRPRFPPPFPIPRIPRIP-OH
- Chemical Formula:C188H300N62O34
- Sequence length:32
- Extinction coefficient:1280 M-1cm-1
- Mw average:3972.78
- Theoretical pI:13
- Data Source:Peptide Property Calculator
GRAVY = grand average of hydropathy
Red: Hydrophobic uncharged residues, like F I L M V W A and P
Blue: Basic residues, like R K H and N-terminal -NH2
Green: Acidic residues, like D E and C-terminal -COOH
Black: Polar uncharged residues, like G S T C N Q and Y
Related Products / Services
• Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.
• Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.
• Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.
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