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Product Name
TRMT61A Polyclonal Antibody
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Description
Polyclonal antibody to TRMT61A
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
TRMT61A antibody; C14orf172 antibody; GCD14 antibody; Gcd14p antibody; TRM61 antibody; hTRM61 antibody; tRNA methyltransferase 61A antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-289 of human TRMT61A (NP_689520.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:1000 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - TRMT61A Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TRMT61A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
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Background
Catalytic subunit of tRNA (adenine-N(1)-)-methyltransferase, which catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA. Catalytic subunit of mRNA N(1)-methyltransferase complex, which mediates methylation of adenosine residues at the N(1) position of a small subset of mRNAs: N(1) methylation takes place in tRNA T-loop-like structures of mRNAs and is only present at low stoichiometries.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"