TREM1 antibody

Cat.#: 116356

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Product Information

  • Product Name
    TREM1 antibody
  • Documents
  • Description
    TREM1 Rabbit Polyclonal antibody. Positive WB detected in PC-3 cells, A549 cells, HepG2 cells, human liver tissue, human spleen tissue, mouse lung tissue. Positive IF detected in HepG2 cells. Positive FC detected in HeLa cells. Observed molecular weight by Western-blot: 30 kDa
  • Tested applications
    ELISA, WB, IF, FC
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    CD354 antibody; TREM 1 antibody; TREM1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of TREM1 recombinant protein (Accession Number: NM_018643). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IF: 1:10-1:100

  • Validations

    PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:116356(TREM1 antibody) at dilution of 1:400

    PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:116356(TREM1 antibody) at dilution of 1:400

    Immunofluorescent analysis of HepG2 cells, using TREM1 antibody Catalog No:116356 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

    Immunofluorescent analysis of HepG2 cells, using TREM1 antibody Catalog No:116356 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

    1X10^6 HeLa cells were stained with 0.2ug TREM1 antibody (Catalog No:116356, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

    1X10^6 HeLa cells were stained with 0.2ug TREM1 antibody (Catalog No:116356, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

  • Background
    Triggering receptor expressed on myeloid cells 1 (TREM1, also known as CD354) is a member of the immunoglobulin superfamily, which is expressed on neutrophils and monocytes. TREM1 plays a role in the innate immune system. TREM1 amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"