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Product Name
Recombinant TPK1 protein (His tag)
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Description
Recombinant Human Thiamine Pyrophosphokinase 1 (hTPK1) fragment (residues 1-243) is a high-purity protein designed for structural and biochemical studies. This stable and soluble fragment retains key functional regions, enabling researchers to investigate thiamine phosphorylation and its role in cofactor biosynthesis and metabolic regulation.
Thiamine pyrophosphokinase 1 (TPK1) catalyzes the phosphorylation of thiamine to thiamine pyrophosphate (TPP) using UTP, linking TPP biosynthesis to pyrimidine metabolism. By producing TPP, a crucial cofactor for the mitochondrial pyruvate dehydrogenase complex, TPK1 indirectly regulates pyruvate oxidation and lipogenesis. While the enzyme can utilize ATP and CTP for thiamine phosphorylation in vitro, this occurs with significantly lower efficiency and lacks established physiological relevance. The full-length protein's role in metabolic pathways underscores its importance in cellular energy metabolism and cofactor biosynthesis.
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Protein name
Thiamine pyrophosphokinase 1
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Uniprot ID
Q9H3S4
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Gene Name
TPK1
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
DNA encoding 1-243 aa (Q9H3S4) were fused with 6His tag.
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Protein Species
Human
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Molecular weight
Predictes a molecular mass of 28.92 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 29 kDa band.
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Purity
>88%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"