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Product Name
TOLLIP antibody
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Description
TOLLIP Rabbit Polyclonal antibody. Positive IHC detected in human gliomas tissue. Positive IF detected in PC-3 cells. Positive WB detected in PC-3 cells, HEK-293 cells, human liver tissue, mouse brain tissue, rat brain tissue. Observed molecular weight by Western-blot: 30 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ33531 antibody; IL 1RAcPIP antibody; toll interacting protein antibody; TOLLIP antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TOLLIP recombinant protein (Accession Number: NM_019009). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:116167(TOLLIP antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:116167(TOLLIP antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of PC-3 cells using Catalog No:116167(TOLLIP Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
TOLLIP is a component of the signaling pathway of IL-1 and Toll-like receptors. It controls the magnitude of inflammatory cytokine production in response to IL-1B and LPS.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"