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Product Name
TIMP4 Polyclonal Antibody
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Description
Polyclonal antibody to TIMP4
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
TIMP4 antibody; metalloproteinase inhibitor 4 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-224 of human TIMP4 (NP_003247.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:10 - 1:100 -
Validations
Western blot - TIMP4 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TIMP4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - TIMP4 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using TIMP4 antibody . Blue: DAPI for nuclear staining.
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Background
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"