TGF-beta1 affinitive peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

• HSNGLPL peptide is a TGF-β1-binding peptide with unique properties and significant potential in the field of biomaterials and tissue engineering.
• This peptide can be grafted onto polyurethane surface via copper-catalyzed azide–alkyne cycloaddition (CuAAC) click reaction. It plays a crucial role in stabilizing the spatial conformation of TGF-β1 after adhesion. When TGF-β1 adheres to the peptide-grafted polyurethane surface, it forms a loose and diffuse layer, in contrast to the compact and rigid layer on the unmodified polyurethane surface. This indicates that the peptide helps maintain the native structure of TGF-β1, which is beneficial for its bioactivity. The affinitive interaction between HSNGLPL and TGF-β1 also slows down the adsorption process of TGF-β1 on the surface. In vivo animal experiments have shown that the peptide-modified polyurethane with TGF-β1 adsorbed elicits earlier immune reactions and tissue regenerations compared to the unmodified polyurethane, confirming that the peptide can preserve the bioactivity of TGF-β1 on the polymer surface. Overall, HSNGLPL peptide offers a promising approach for improving the performance of biomaterials in tissue engineering applications by enhancing the stability and bioactivity of growth factors like TGF-β1.

• Xiao M, Xiao J, Wu G, Ke Y, Fang L, Deng C, Liao H. Anchoring TGF-β1 on biomaterial surface via affinitive interactions: Effects on spatial structures and bioactivity. Colloids Surf B Biointerfaces. 2018 Jun 1;166:254-261. doi: 10.1016/j.colsurfb.2018.02.059. Epub 2018 Feb 28. PMID: 29602080.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.