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Product Name
TCOF1 antibody
- Documents
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Description
TCOF1 Rabbit Polyclonal antibody. Positive IHC detected in human lymphoma tissue. Positive IF detected in MCF-7 cells. Positive WB detected in HeLa cells, Jurkat cells. Observed molecular weight by Western-blot: 220 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
MFD1 antibody; TCOF1 antibody; treacle antibody; Treacle protein antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TCOF1 recombinant protein (Accession Number: NM_001008657). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115914(TCOF1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:115914(TCOF1 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of MCF-7 cells, using TCOF1 antibody Catalog No:115914 at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
TCOF1 represents Treacher Collins-Franceschetti syndrome 1
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References
- Ciccia A, Huang JW, Izhar L, Sowa ME, Harper JW, Elledge SJ. Treacher Collins syndrome TCOF1 protein cooperates with NBS1 in the DNA damage response. Proceedings of the National Academy of Sciences of the United States of America. 111(52):18631-6. 2014.
- Werner A, Iwasaki S, McGourty CA. Cell-fate determination by ubiquitin-dependent regulation of translation. Nature. 525(7570):523-7. 2015.
- Park A, Won ST, Pentecost M, Bartkowski W, Lee B. CRISPR/Cas9 allows efficient and complete knock-in of a destabilization domain-tagged essential protein in a human cell line, allowing rapid knockdown of protein function. PloS one. 9(4):e95101. 2014.
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