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Product Name
Syntaxin 8 antibody
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Description
Syntaxin 8 Rabbit Polyclonal antibody. Positive IP detected in A375 cells. Positive WB detected in human heart tissue, A375 cells, human brain tissue, human kidney tissue, human liver tissue, human lung tissue, human placenta tissue, mouse pancreas tissue. Positive IF detected in HepG2 cells. Positive FC detected in HepG2 cells. Observed molecular weight by Western-blot: 27 kDa
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Tested applications
ELISA, IF, WB, FC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CARB antibody; STX8 antibody; syntaxin 8 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Syntaxin 8 recombinant protein (Accession Number: NM_004853). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:115801(STX8 antibody) at dilution of 1:500
1X10^6 HepG2 cells were stained with 0.2ug STX8 antibody (Catalog No:115801, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
Immunofluorescent analysis of HepG2 cells, using STX8 antibody Catalog No:115801 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-STX8 (IP:Catalog No:115801, 4ug; Detection:Catalog No:115801 1:500) with A375 cells lysate 3600ug.
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Background
Syntaxin 8 (STX8), also known as CIP-1-associated regulator of cyclin B (CARB ), is a protein belongs to the syntaxin family. The gene of STX8 maps to chromosomal band 17p12 and it encodes a 236-amino-acid protein containing 1 t-SNARE coiled-coil homology domain. STX8 mRNA is broadly expressed in multiple human tissues, including heart, brain, kidney, liver, lung, placenta, skeletal muscle, spleen and pancreas (PMID: 10198254). STX8 is involved in protein trafficking from early to late endosomes via vesicle fusion and exocytosis.
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