ssK36 peptide

ssK36 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-APRFGGVKRPNRYRP-OH

H-Ala-Pro-Arg-Phe-Gly-Gly-Val-Lys-Arg-Pro-Asn-Arg-Tyr-Arg-Pro-OH

15

95.17%

C₇₉H₁₂₇N₂₉O₁₈

1771.03

Synthetic

Powder

ssK36, or ssK36 (29–43), is a SET-domain containing protein 2 (SETD2) super substrate. SETD2, also called KMT3A, was first identified as huntingtin interacting protein 1 (HYPB, HIP-1) and can add up to three methyl groups to K36 of histone H39 (H3K36). ssK36 (29-43 has been derived from the currently used SETD2 substrate peptide H3K36 (29-43) but is methylated about 290-fold faster, making it a super substrate. The increased methylation of ssK36 is due to an increase in the rate of catalysis and only to a smaller degree to improved binding.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Schuhmacher et al (2020) Sequence specificity analysis of the SETD2 protein lysine methyltransferase and discovery of a SETD2 super-substrate. Commun. Biol. 3(1) 511 PMID: 32939018

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.