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Product Name
SRP9 antibody
- Documents
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Description
SRP9 Rabbit Polyclonal antibody. Positive IP detected in HepG2 cells. Positive WB detected in HepG2 cells, A2780 cells, MCF7 cells, mouse colon tissue, SKOV-3 cells. Positive IHC detected in human prostate cancer tissue. Observed molecular weight by Western-blot: 10 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ALURBP antibody; SRP9 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SRP9 recombinant protein (Accession Number: NM_003133). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:115595(SRP9 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:115595(SRP9 antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-SRP9 (IP:Catalog No:115595, 3ug; Detection:Catalog No:115595 1:500) with HepG2 cells lysate 3600ug.
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Background
Signal recognition particle 9(SRP9) is component of the signal recognition particle (SRP), which is a ribonucleoprotein complex that mediates the targeting of proteins to the rough endoplasmic reticulum (ER). SRP9 form a heterodimer with SRP14, which involves in arrest of the elongation of the nescent chains during targeting to ensure efficient translocation of the preprotein.
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References
- Rho JH, Qin S, Wang JY, Roehrl MH. Proteomic expression analysis of surgical human colorectal cancer tissues: up-regulation of PSB7, PRDX1, and SRP9 and hypoxic adaptation in cancer. Journal of proteome research. 7(7):2959-72. 2008.
- Piazzon N, Schlotter F, Lefebvre S. Implication of the SMN complex in the biogenesis and steady state level of the signal recognition particle. Nucleic acids research. 41(2):1255-72. 2013.
- Hessel EV, de Wit M, Wolterink-Donselaar IG. Identification of Srp9 as a febrile seizure susceptibility gene. Annals of clinical and translational neurology. 1(4):239-50. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"