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Product Name
SPTLC1 Polyclonal Antibody
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Description
Polyclonal antibody to SPTLC1
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
SPTLC1 antibody; HSAN1 antibody; HSN1 antibody; LBC1 antibody; LCB1 antibody; SPT1 antibody; SPTI antibody; serine palmitoyltransferase 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 37-310 of human SPTLC1 (NP_006406.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - SPTLC1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SPTLC1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - SPTLC1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human kidney using SPTLC1 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SPTLC1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse kidney using SPTLC1 Antibody at dilution of 1:100 (40x lens).
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Background
Serine palmitoyltransferase (SPT). The heterodimer formed with SPTLC2 or SPTLC3 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. The SPTLC1-SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference. Required for adipocyte cell viability and metabolic homeostasis (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"