Recombinant SPOP protein (His tag)

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Recombinant Human SPOP Protein Fragment (1-374) is a high-purity, functional fragment encompassing the full MATH and BTB domains of Speckle-type POZ protein. This product is an essential tool for studying ubiquitin ligase complex formation, substrate recognition, and protein degradation pathways in cellular regulation.

Speckle-type POZ protein (SPOP) is a critical component of cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes that mediate substrate ubiquitination, typically leading to proteasomal degradation. SPOP targets multiple key regulatory proteins including BRMS1, DAXX, PDX1/IPF1, GLI transcription factors, BET proteins, and interferon regulatory factor 1, playing fundamental roles in transcriptional regulation, DNA replication, viral defense, and spermatogenesis. The protein localizes primarily to nuclear speckles and the nucleus, with some cytoplasmic presence, where it functions as a substrate adaptor that recognizes specific degron motifs in target proteins. The full-length protein's ability to form homodimers enhances its ubiquitin ligase activity, making it a crucial regulator of protein homeostasis and cellular signaling pathways.