Recombinant SPIR1 protein (His tag)

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Recombinant Human Protein spire homolog 1 (Spir-1) Fragment (residues 37-387) is a high-purity protein designed for studying actin nucleation and intracellular transport mechanisms. This fragment encompasses the critical KIND and WH2 domains, making it an ideal tool for investigating cytoskeletal dynamics, vesicle transport, and asymmetric cell division in research applications.

Protein spire homolog 1 (Spir-1), encoded by the SPIRE1 gene in humans, functions as an actin nucleation factor that promotes actin filament assembly and remains associated with the pointed end of filaments. It plays essential roles in intracellular vesicle transport along actin fibers, linking cytoskeleton dynamics with cellular trafficking. Spir-1 is critical for asymmetric spindle positioning and cell division during meiosis, particularly in female germ cells where it supports cleavage furrow formation and polar body extrusion. Additionally, it contributes to nuclear actin filament assembly in response to DNA damage, facilitating chromatin movement and repair, and promotes innate immune signaling by aiding IRF3 phosphorylation downstream of MAVS. Subcellularly, Spir-1 localizes to the cytoplasm, cytoskeleton, perinuclear region, cell membrane, and cytoplasmic vesicle membranes, with presence at cleavage furrows and Rab11-positive puncta, underscoring its multifaceted roles in cellular organization and signaling pathways.

37-387