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Product Name
SNAP29 antibody
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Description
SNAP29 Rabbit Polyclonal antibody. Positive WB detected in human kidney tissue, HEK-293 cells, human liver tissue, Jurkat cells. Positive IP detected in Jurkat cells. Positive IF detected in HEK-293 cells. Positive IHC detected in human kidney tissue, human stomach tissue. Observed molecular weight by Western-blot: 29 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CEDNIK antibody; FLJ21051 antibody; SNAP 29 antibody; SNAP29 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SNAP29 recombinant protein (Accession Number: NM_004782). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
human kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:115447(SNAP29 antibody) at dilution of 1:500
IP Result of anti-SNAP29 (IP:Catalog No:115447, 4ug; Detection:Catalog No:115447 1:600) with Jurkat cells lysate 3200ug.
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:115447(SNAP29 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:115447(SNAP29 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed HEK-293 cells using Catalog No:115447(SNAP29 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
SNAP29 represents synaptosomal-associated protein, 29kDa
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References
- Lu Q, Insinna C, Ott C. Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation. Nature cell biology. 17(4):531. 2015.
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