SMN antibody

Cat.#: 115392

Size:

Special Price 239.0 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    SMN antibody
  • Documents
  • Description
    SMN Rabbit Polyclonal antibody. Positive IP detected in HEK-293. Positive WB detected in HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human kidney tissue, human heart tissue. Observed molecular weight by Western-blot: 38kd
  • Tested applications
    ELISA, WB, IHC, IF, IP
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    C BCD541 antibody; Component of gems 1 antibody; FLJ76644 antibody; Gemin 1 antibody; SMN antibody; SMN1 antibody; SMN1 antibody; SMN antibody; SMN2 antibody; SMNC antibody; SMNT antibody; Survival motor neuron protein antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of SMN recombinant protein (Accession Number: NM_022875). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:115392(SMN2 antibody) at dilution of 1:800

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:115392(SMN2 antibody) at dilution of 1:800

    IP result of anti-SMN2(Catalog No:115392 for IP and Detection).

    IP result of anti-SMN2(Catalog No:115392 for IP and Detection).

    Immunohistochemical of paraffin-embedded human kidney using Catalog No:115392(SMN2 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human kidney using Catalog No:115392(SMN2 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human kidney using Catalog No:115392(SMN2 antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human kidney using Catalog No:115392(SMN2 antibody) at dilution of 1:100 (under 40x lens)

    Immunofluorescent analysis of HepG2 cells, using SMN2 antibody Catalog No:115392 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of HepG2 cells, using SMN2 antibody Catalog No:115392 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Background
    Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of anterior horn cells in the spinal cord and concomitant symmetrical muscle weakness and atrophy (PMID: 16364894 ). SMA is caused by deletion or mutations of the survival motor neuron (SMN1) gene. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional (PMID: 17355180). A large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein (PMID: 10369862). The level of SMN protein correlates with phenotypic severity of SMA.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"