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Product Name
Recombinant SMD1 protein (His tag)
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Description
Recombinant Human Small nuclear ribonucleoprotein Sm D1 (Sm-D1) Fragment (residues 1-87) is a high-purity protein designed for studying spliceosome assembly and pre-mRNA splicing mechanisms. This fragment fully encompasses the functional Sm domain, making it an ideal tool for investigating domain-specific interactions in snRNP complexes.
Small nuclear ribonucleoprotein Sm D1 (SNRPD1) serves as a core component of spliceosomal U1, U2, U4, and U5 small nuclear ribonucleoproteins (snRNPs), which are fundamental building blocks of the spliceosome. It participates in both the pre-catalytic B complex and activated C complex stages of spliceosome assembly and is also involved in the minor spliceosome for U12-type intron splicing. The protein may function as a charged scaffold that promotes snRNP assembly and strengthens snRNP-snRNP interactions through electrostatic contacts with RNA. Cellular localization studies show Sm-D1 is initially assembled in the cytosol before being transported to the nucleus via S
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Protein name
Small nuclear ribonucleoprotein Sm D1
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Uniprot ID
P62314
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Gene Name
SNRPD1
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
DNA encoding 1-87 aa (P62314) were fused with N-terminal 6His tag.
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Sequence
MNHKVHHHHH HGGSMKLVRF LMKLSHETVT IELKNGTQVH GTITGVDVSM NTHLKAVKMT LKNREPVQLE TLSIRGNNIR YFILPDSLPL DTLLVDVEPK V
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Protein Species
Human
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Molecular weight
Predictes a molecular mass of 11.46 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 13 kDa band.
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Purity
>90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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Validations
Recombinant SMD1 protein (His tag) SDS-PAGE
M: Marker; Lane 1: SMD1; Lane 2: BSA
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