SMAP-29 peptide

SMAP-29 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

H-RKLRRLKRKIAHKVKKY-OH

H-Arg-Lys-Leu-Arg-Arg-Leu-Lys-Arg-Lys-Ile-Ala-His-Lys-Val-Lys-Lys-Tyr-OH

17

95.8%

C₁₀₁H₁₈₅N₃₇O₁₉

2221.78

Synthetic

Powder

SMAP-29 (N-terminus sheep myeloid peptide of 29 amino acids) has a minimum inhibitory concentration (MIC) at 0.3–5.2 µM for Gram-positive and 0.1–9.8 µM for Gram-negative bacteria. It has been demonstrated that SMAP-29 fusion can significantly improve the bactericidal activity of endolysin and make it especially efficient against Gram-negative bacteria, including multidrug-resistant strains.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Antonova NP, Vasina DV, Rubalsky EO, Fursov MV, Savinova AS, Grigoriev IV, Usachev EV, Shevlyagina NV, Zhukhovitsky VG, Balabanyan VU, Potapov VD, Aleshkin AV, Makarov VV, Yudin SM, Gintsburg AL, Tkachuk AP, Gushchin VA. Modulation of Endolysin LysECD7 Bactericidal Activity by Different Peptide Tag Fusion. Biomolecules. 2020 Mar 12;10(3):440. doi: 10.3390/biom10030440. PMID: 32178329; PMCID: PMC7175214.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.