-
Product Name
Recombinant SMAD6 protein (His tag)
- Documents
-
Description
Recombinant Human SMAD6 Fragment (aa 329-496) is a high-purity protein designed for studying the MH2 domain's role in regulating TGF-beta and BMP signaling pathways. This fragment serves as an essential tool for investigating inhibitory Smad mechanisms and their impact on cellular signaling and inflammation.
SMAD6, or Mothers against decapentaplegic homolog 6, is an inhibitory Smad (i-Smad) that negatively regulates signaling mediated by the transforming growth factor-beta (TGF-beta) superfamily receptors. It functions by competing with SMAD4 for binding to receptor-activated SMAD1, thereby blocking the BMP-SMAD1 signaling pathway. Additionally, SMAD6 acts as a mediator of TGF-beta and BMP anti-inflammatory activities through its suppression of IL1R-TLR signaling, preventing NF-kappa-B activation and the expression of pro-inflammatory genes. The protein is localized to the nucleus, where it binds to regulatory elements in target promoter regions, underscoring its critical role in transcriptional regulation and cellular homeostasis.
-
Protein name
Mothers against decapentaplegic homolog 6
-
Uniprot ID
O43541
-
Gene Name
SMAD6; MADH6
-
Source/Expression Host
E. coli
-
Expression Plasmid/cDNA
DNA encoding 329-496 aa (O43541) were fused with 6His tag.
-
Protein Species
Human
-
Molecular weight
Predictes a molecular mass of 20.59 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 21 kDa band.
-
Purity
>90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
-
Activity
Not tested.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"