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Product Name
Recombinant SMAD2 protein (His tag)
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Description
Recombinant Human SMAD2 (MAD homolog 2) Fragment (aa 262-467): A high-purity protein fragment encompassing the complete MH2 domain, designed for targeted investigation of TGF-beta and activin signaling pathways, transcriptional regulation, and intracellular signal transduction mechanisms in cellular and biochemical assays.
SMAD2 (Mothers against decapentaplegic homolog 2) is a receptor-regulated SMAD (R-SMAD) that functions as a crucial intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. It binds to TRE promoter elements and, upon complex formation with SMAD4, activates transcription of TGF-beta-regulated genes, contributing to processes such as odontoblastic differentiation. SMAD2 also positively regulates PDPK1 kinase activity and may act as a tumor suppressor in colorectal carcinoma. The protein localizes to both the cytoplasm and nucleus, with nuclear translocation occurring upon TGF-beta stimulation through complex formation with SMAD4 or IPO7, followed by nuclear export after dephosphorylation by PPM1A. This dynamic subcellular shuttling underscores its role as a key mediator in TGF-beta signaling pathways.
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Protein name
Mothers against decapentaplegic homolog 2
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Uniprot ID
Q15796
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Gene Name
SMAD2; MADH2; MADR2
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
DNA encoding 262-467 aa (Q15796) were fused with 6His tag.
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Protein Species
Human
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Molecular weight
Predictes a molecular mass of 24.88 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 25 kDa band.
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Purity
>86%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"