Recombinant SKI2 protein (His tag)

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Recombinant Human Superkiller complex protein 2 (Ski2) Fragment (aa 55-195) - A highly stable and soluble protein fragment ideal for structural and biochemical studies of this essential RNA helicase component involved in mRNA decay and quality control pathways.

Superkiller complex protein 2 (Ski2) is a helicase component of the SKI complex, a multiprotein complex that plays a critical role in assisting the RNA-degrading exosome during mRNA decay and quality-control pathways. The SKI complex catalyzes mRNA extraction from 80S ribosomal complexes in the 3'-5' direction and channels mRNA to the cytosolic exosome for degradation. This process is essential for extracting mRNA from stalled ribosomes, allowing binding of the Pelota-HBS1L complex and subsequent ribosome disassembly by ABCE1 for ribosome recycling. Additionally, in the nucleus, the SKI complex associates with transcriptionally active genes in a PAF1 complex-dependent manner. Ski2 is localized in both the nucleus and cytoplasm, reflecting its dual roles in nuclear transcription-associated processes and cytoplasmic mRNA degradation pathways. The recombinant fragment (amino acids 55-195) represents a stable, soluble unit selected for structural and functional studies of this important RNA helicase.