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Product Name
SIRT5 antibody
- Documents
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Description
SIRT5 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, mouse liver tissue. Positive IP detected in mouse liver tissue. Observed molecular weight by Western-blot: 33kd
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ36950 antibody; SIR2 like protein 5 antibody; SIR2L5 antibody; SIRT5 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SIRT5 recombinant protein (Accession Number: NM_001376801). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115246(SIRT5 antibody) at dilution of 1:400
IP Result of anti-SIRT5 (IP:Catalog No:115246, 4ug; Detection:Catalog No:115246 1:500) with mouse liver tissue lysate 6000ug.
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Background
SIRT5, a member fo sirtuin family that is a mitochondrial matrix NAD(+)-dependent deacetylase and mono-ADP-ribosyltransferases. Sirtuins play important role in various cellular processed, such as aging, gene silencing and metabolism. SIRT5 mainly locates in . It is an efficient protein lysine desuccinylase and demalonylase. During prolonged fasting, it can activate CPS1, the committed and regulated enzyme of the urea cycle, and contribute to the regulation of blood ammonia levels.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"