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Product Name
SHMT1 antibody
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Description
SHMT1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive WB detected in human kidney tissue, HeLa cells, HepG2 cells, human liver tissue, mouse kidney tissue. Observed molecular weight by Western-blot: 53 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CSHMT antibody; Serine methylase antibody; SHMT antibody; SHMT1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SHMT1 recombinant protein (Accession Number: NM_004169). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:10-1:100
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Validations
human kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:115276(SHMT1 antibody) at dilution of 1:500
Immunofluorescent analysis of HepG2 cells using Catalog No:115276(SHMT1 Antibody) at dilution of 1:25 and Alexa Fluor 594-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
SHMT1 is the cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. Alternative splicing of this gene results in 2 transcript variants encoding 2 different isoforms. This antibody can detect both 49kd and 53 kd isoforms in western blotting.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"