Recombinant SHH protein (His tag)

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This product is a recombinant fragment of the Sonic hedgehog protein (SHH) from Homo sapiens, encompassing amino acids 24-390. This fragment includes the N-terminal signaling domain (24-197) and is designed for structural and biochemical studies, offering a stable and soluble unit for research applications.

The full-length Sonic hedgehog protein (SHH) is a critical morphogen involved in various developmental processes, including neural tube and somite patterning, limb bud development, and axon guidance. It undergoes autoproteolysis in the endoplasmic reticulum, producing the N-terminal signaling domain (ShhN) and a C-terminal fragment (ShhC), which is degraded. ShhN, dually lipidated, acts as a ligand that binds to the patched receptor (PTCH1) and modulates smoothened (SMO) activity to regulate target gene transcription. In the absence of SHH, PTCH1 represses SMO signaling. The protein localizes to the endoplasmic reticulum and Golgi apparatus membranes during processing, and the mature ShhN is anchored to the cell membrane, with mechanisms like interaction with DISP1 and SCUBE2 facilitating its solubilization and distribution.