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Product Name
RQCD1 antibody
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Description
RQCD1 Rabbit Polyclonal antibody. Positive WB detected in mouse thymus tissue, HepG2 cells, MCF-7 cells. Positive IP detected in MCF-7 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human small intestine tissue. Observed molecular weight by Western-blot: 34-40 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CNOT9 antibody; Rcd 1 antibody; RCD1 antibody; RCD1+ antibody; RQCD1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RQCD1 recombinant protein (Accession Number: BC007102). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse thymus tissue were subjected to SDS PAGE followed by western blot with Catalog No:114918(RQCD1 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human small intestine slide using Catalog No:114918(RQCD1 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human small intestine slide using Catalog No:114918(RQCD1 Antibody) at dilution of 1:50
Immunofluorescent analysis of HepG2 cells using Catalog No:114918 (RQCD1 Antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-RQCD1 (IP:Catalog No:114918, 4ug; Detection:Catalog No:114918 1:1000) with MCF-7 cells lysate 1280ug.
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Background
RQCD1 represents RCD1 required for cell differentiation1 homolog (S. pombe)
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References
- Mathys H, Basquin J, Ozgur S. Structural and biochemical insights to the role of the CCR4-NOT complex and DDX6 ATPase in microRNA repression. Molecular cell. 54(5):751-65. 2014.
- Chen Y, Boland A, Kuzuoğlu-Öztürk D. A DDX6-CNOT1 complex and W-binding pockets in CNOT9 reveal direct links between miRNA target recognition and silencing. Molecular cell. 54(5):737-50. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"