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Product Name
RNPEP antibody
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Description
RNPEP Rabbit Polyclonal antibody. Positive IP detected in A549 cells. Positive WB detected in HeLa cells, A549 cells, HepG2 cells. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 72 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Aminopeptidase B antibody; AP B antibody; APB antibody; Arginine aminopeptidase antibody; Arginyl aminopeptidase antibody; DKFZp547H084 antibody; RNPEP antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RNPEP recombinant protein (Accession Number: NM_020216). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114771(RNPEP antibody) at dilution of 1:500
IP Result of anti-RNPEP (IP:Catalog No:114771, 4ug; Detection:Catalog No:114771 1:500) with A549 cells lysate 2000ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:114771(RNPEP Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
RNPEP represents arginyl aminopeptidase (aminopeptidase B)
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