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  • RNF4 antibody

RNF4 antibody

Cat.#: 114759

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Special Price 239.0 USD

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Product Information

  • Product Name
    RNF4 antibody
  • Documents
  • Description
    RNF4 Rabbit Polyclonal antibody. Positive IP detected in PC-3 cells. Observed molecular weight by Western-blot: 30 kDa
  • Tested applications
    ELISA, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    E3 ubiquitin ligase RNF4 antibody; Protein SNURF antibody; RES4 26 antibody; ring finger protein 4 antibody; RNF4 antibody; SNURF antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of RNF4 recombinant protein (Accession Number: BC031935). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    IP: 1:200-1:1000

  • Validations

    IP Result of anti-RNF4 (IP:Catalog No:114759, 4ug; Detection:Catalog No:114759 1:300) with PC-3 cells lysate 3200ug.

    IP Result of anti-RNF4 (IP:Catalog No:114759, 4ug; Detection:Catalog No:114759 1:300) with PC-3 cells lysate 3200ug.

  • Background
    RNF4 is a E3 ubiquitin-protein ligase that binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. It regulates the degradation of several proteins including PML and PEA3[PMID:18408734]. It also participates in chromosome alignment and spindle assembly, and regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. In addition, RNF4 can regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1 [PMID:20026589]. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation [PMID:11319220]. RNF4 exist as two isoforms. Isoform one is a 30 kDa protein and isoform two is a about 15 kDa protein.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"