RNF4 Rabbit Polyclonal antibody. Positive IP detected in PC-3 cells. Observed molecular weight by Western-blot: 30 kDa
Human,Mouse,Rat; other species not tested.
E3 ubiquitin ligase RNF4 antibody; Protein SNURF antibody; RES4 26 antibody; ring finger protein 4 antibody; RNF4 antibody; SNURF antibody
This antibody was obtained by immunization of RNF4 recombinant protein (Accession Number: BC031935). Purification method: Antigen affinity purified.
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Store at -20℃. DO NOT ALIQUOT
IP Result of anti-RNF4 (IP:Catalog No:114759, 4ug; Detection:Catalog No:114759 1:300) with PC-3 cells lysate 3200ug.
RNF4 is a E3 ubiquitin-protein ligase that binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. It regulates the degradation of several proteins including PML and PEA3[PMID:18408734]. It also participates in chromosome alignment and spindle assembly, and regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. In addition, RNF4 can regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1 [PMID:20026589]. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation [PMID:11319220]. RNF4 exist as two isoforms. Isoform one is a 30 kDa protein and isoform two is a about 15 kDa protein.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"