Recombinant RHOA protein (His tag)

• DataSheet
• Material Safety Data Sheets (MSDS)

Recombinant Transforming protein RhoA (EC 3.6.5.2), derived from Homo sapiens, is a high-purity fragment (amino acids 1-190) designed for structural and biochemical studies. This stable, soluble unit retains key functional regions, enabling researchers to investigate RhoA's role in cytoskeletal dynamics, cell migration, and signaling pathways with reliability and precision.

RhoA is a small GTPase that cycles between active GTP-bound and inactive GDP-bound states, primarily regulating cytoskeleton organization, cell migration, and cell cycle progression. It facilitates signal transduction linking plasma membrane receptors to focal adhesion and actin stress fiber assembly, and is essential for processes such as cleavage furrow formation during cytokinesis, apical junction establishment in keratinocytes, and microtubule stabilization via the MEMO1-RHOA-DIAPH1 pathway. RhoA also modulates potassium channel activity and platelet alpha-granule release, and serves as a target for microbial effectors like Yersinia pestis yopT peptidase. Subcellularly, it localizes to the cell membrane, cytoplasm, cytoskeleton, cleavage furrow, midbody, and nucleus, with dynamic translocation to sites like the equatorial cortex during anaphase, underscoring its broad involvement in cellular structure and function.