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Product Name
Recombinant RFC4 protein (His tag)
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Description
Introducing the recombinant human Replication factor C subunit 4 (RFC4) fragment (residues 37-363), a highly stable and soluble protein unit ideal for investigating DNA replication mechanisms. This 327-amino acid fragment retains key structural features, making it perfect for structural biology studies and biochemical assays exploring RFC complex assembly and PCNA loading dynamics.
Replication factor C subunit 4 is an essential component of the RFC complex in Homo sapiens, which plays a critical role in DNA replication elongation by DNA polymerases δ and ε. The complex facilitates ATP-dependent loading of proliferating cell nuclear antigen (PCNA) onto primed DNA templates, a fundamental step in processive DNA synthesis. RFC4 functions as a structural scaffold that enables proper assembly of other RFC subunits. This nuclear-localized protein is vital for maintaining genomic integrity, with implications for understanding cell division and DNA repair pathways. The selected fragment (37-363) represents a computationally optimized stable domain that preserves the protein's core structural integrity while enhancing experimental utility.
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Protein name
Replication factor C subunit 4
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Uniprot ID
P35249
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Gene Name
RFC4
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
DNA encoding 37-363 aa (P35249) were fused with 6His tag.
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Protein Species
Human
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Molecular weight
Predictes a molecular mass of 37.69 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 40 kDa band.
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Purity
>89%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"