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Product Name
Human FLT-3/CD135/FLK-2 (His Tag) recombinant protein
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Description
Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.
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Protein name
Receptor-type tyrosine-protein kinase FLT3
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Protein short names
FLT-3; FLK-2; FLK2; CD135; RP11-153M24.3; WMFL; MGC124378; FLT3; MGC124377; B230315G04; STK1; LY72
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Uniprot ID
P36888
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Gene Name
FLT3; CD135; FLK2; STK1
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the extracellular domain of human FLT3 (NP_004110.2) (Met 1-Asn 541) was expressed, with a C-terminal polyhistidine tag.
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Protein Species
Human
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Molecular weight
The recombinant human FLT3 consists of 526 amino acids and has a predicted molecular mass of 59.7 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 85-95 kDa due to glycosylation.
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Purity
> 85 % as determined by SDS-PAGE
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Activity
Measured by its ability to bind biotinylated human FLT3L-His (Cat:500686) in a functional ELISA.
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Validations
Human FLT-3 / CD135 / FLK-2 Protein (His Tag) SDS-PAGE
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