-
Product Name
Recombinant RDHE2 protein (His tag)
- Documents
-
Description
Recombinant Human Epidermal Retinol Dehydrogenase 2 (EPHD-2) Fragment (residues 32-269) is a high-purity protein designed for structural and biochemical studies. This 238-amino acid fragment retains the oxidoreductase activity critical for converting all-trans-retinol to all-trans-retinaldehyde, providing a stable and soluble unit ideal for research applications.
Epidermal Retinol Dehydrogenase 2 (EPHD-2), encoded by the SDR16C5 gene, is an NAD-dependent oxidoreductase active in both oxidative and reductive directions. It specifically catalyzes the oxidation of all-trans-retinol to all-trans-retinaldehyde, a key step in retinoid metabolism, but shows no activity with 11-cis-retinoids. The full-length protein is localized to the endoplasmic reticulum membrane as a multi-pass membrane protein, where it participates in critical metabolic pathways. This recombinant fragment represents a computationally optimized soluble domain that maintains functional relevance while enabling experimental accessibility.
-
Protein name
Epidermal retinol dehydrogenase 2
-
Uniprot ID
Q8N3Y7
-
Gene Name
SDR16C5; RDHE2
-
Source/Expression Host
E. coli
-
Expression Plasmid/cDNA
DNA encoding 32-269 aa (Q8N3Y7) were fused with 6His tag.
-
Protein Species
Human
-
Molecular weight
Predictes a molecular mass of 27.61 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 28 kDa band.
-
Purity
>90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
-
Activity
Not tested.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"