Recombinant RAN protein (His tag)

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Recombinant GTP-binding nuclear protein Ran (Ras-related nuclear protein) from Drosophila melanogaster, encompassing the full-length fragment (amino acids 1-216). This product includes the complete Small GTPase Ran-type domain, making it an ideal tool for investigating nucleocytoplasmic transport, mitotic spindle regulation, and GTPase-driven molecular mechanisms in cellular and developmental studies.

GTP-binding nuclear protein Ran is a crucial GTPase that regulates nucleocytoplasmic transport by cycling between GDP-bound cytoplasmic and GTP-bound nuclear states. It directs the loading and release of cargoes by nuclear import and export receptors, ensuring directional transport of proteins and RNAs. Ran also plays a vital role in mitosis, where its GTP-bound form promotes microtubule assembly at chromosomes, facilitates spindle formation, and supports chromosome segregation. In Drosophila melanogaster, Ran is essential for normal mitotic progression, nuclear envelope assembly, and during oogenesis, it aids in the formation of Nup358-containing granules and nuclear pore complex biogenesis by mediating microtubule-based transport. The protein localizes to the nucleus, nuclear envelope, cytoplasm, and spindle apparatus, highlighting its dynamic involvement in key cellular compartments and processes.