Recombinant RAG1 protein (His tag)

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Recombinant Human RAG1 (97-215) Fragment is a high-purity, stable protein fragment derived from the V(D)J recombination-activating protein 1 (RAG-1), essential for immune receptor gene rearrangement. This 119-amino acid fragment, selected for its solubility and structural integrity, is ideal for biochemical and structural studies, enabling researchers to investigate mechanisms of adaptive immunity development.

The full-length RAG1 protein, encoded by the RAG1 gene in Homo sapiens (UniProt ID: P15918), is a catalytic component of the RAG complex that mediates DNA cleavage during V(D)J recombination—a critical process for generating diverse immunoglobulin and T-cell receptor genes in developing lymphocytes. It functions as both an endonuclease, introducing double-strand breaks at recombination signal sequences to initiate gene segment rearrangement, and an E3 ubiquitin-protein ligase, monoubiquitinating histone H3 to facilitate DNA joining steps. This protein is localized in the nucleus and also contributes to pre-B cell allelic exclusion, ensuring clonal specificity by limiting recombination to a single allele. The provided fragment supports focused research on RAG1's structural and functional properties without domain-specific annotations.