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Product Name
RAB7A antibody
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Description
RAB7A Rabbit Polyclonal antibody. Positive IHC detected in human stomach tissue, human skeletal muscle tissue. Positive IP detected in SH-SY5Y cells. Positive WB detected in SH-SY5Y cells, A431 cells, C6 cells, mouse brain tissue, NIH/3T3 cells. Observed molecular weight by Western-blot: 23 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ20819 antibody; PRO2706 antibody; RAB7 antibody; RAB7A antibody; Ras related protein Rab 7a antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_004637). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with Catalog No:114458(RAB7A antibody) at dilution of 1:800
IP Result of anti-RAB7A (IP:Catalog No:114458, 4ug; Detection:Catalog No:114458 1:300) with SH-SY5Y cells lysate 1600ug.
Immunohistochemistry of paraffin-embedded human stomach tissue slide using Catalog No:114458(RAB7A Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human stomach tissue slide using Catalog No:114458(RAB7A Antibody) at dilution of 1:50 (under 40x lens)
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Background
RAB7A, also named as RAB7, belongs to the small GTPase superfamily and Rab family. It is involved in late endocytic transport. RAB7A contributes to the maturation of phagosomes (acidification). Defects in RAB7A are the cause of Charcot-Marie-Tooth disease type 2B (CMT2B). This antibody is specific to RAB7A.
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References
- Hu X, Yu J, Zhou X. Synergism between upregulation of Rab7 and inhibition of autophagic degradation caused by mycoplasma facilitates intracellular mycoplasma infection. Molecular medicine reports. 9(3):793-800. 2014.
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