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Product Name
RAB3IP/Rabin8 antibody
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Description
RAB3IP/Rabin8 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human pancreas cancer tissue, human pancreas tissue. Positive IP detected in mouse lung tissue. Positive WB detected in human brain tissue, human heart tissue, mouse lung tissue. Observed molecular weight by Western-blot: 56 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ14660 antibody; FLJ22548 antibody; Rab 3A interacting protein antibody; Rab3A interacting protein antibody; RAB3IP antibody; Rabin 3 antibody; RABIN3 antibody; Rabin8 antibody; SSX2 interacting protein antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RAB3IP/Rabin8 recombinant protein (Accession Number: XM_017018777). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:114448(RABIN8 antibody) at dilution of 1:2000
IP Result of anti-RABIN8 (IP:Catalog No:114448, 3ug; Detection:Catalog No:114448 1:1000) with mouse lung tissue lysate 4500ug.
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:114448(RABIN8 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using RAB3IP antibody Catalog No:114448 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
RAB3IP represents RAB3A interacting protein (rabin3)
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References
- Bryant DM, Datta A, Rodríguez-Fraticelli AE, Peränen J, Martín-Belmonte F, Mostov KE. A molecular network for de novo generation of the apical surface and lumen. Nature cell biology. 12(11):1035-45. 2010.
- Parkhitko CA, Favorova CO, Henske EP. Rabin8 Protein Interacts with GTPase Rheb and Inhibits Phosphorylation of Ser235/Ser236 in Small Ribosomal Subunit Protein S6. Acta naturae. 3(3):71-6. 2011.
- Demir K, Kirsch N, Beretta CA. RAB8B is required for activity and caveolar endocytosis of LRP6. Cell reports. 4(6):1224-34. 2013.
- Burke MC, Li FQ, Cyge B. Chibby promotes ciliary vesicle formation and basal body docking during airway cell differentiation. The Journal of cell biology. 207(1):123-37. 2014.
- Zhang B, Zhang T, Wang G. GSK3β-Dzip1-Rab8 cascade regulates ciliogenesis after mitosis. PLoS biology. 13(4):e1002129. 2015.
- Ultanir SK, Hertz NT, Li G. Chemical genetic identification of NDR1/2 kinase substrates AAK1 and Rabin8 Uncovers their roles in dendrite arborization and spine development. Neuron. 73(6):1127-42. 2012.
- Lai YC, Kondapalli C, Lehneck R. Phosphoproteomic screening identifies Rab GTPases as novel downstream targets of PINK1. The EMBO journal. 34(22):2840-61. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"