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Product Name
RAB3B antibody
- Documents
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Description
RAB3B Rabbit Polyclonal antibody. Positive WB detected in human brain tissue. Positive FC detected in HepG2 cells. Positive IHC detected in human colon cancer tissue, human pancreas tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 25kd
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Tested applications
ELISA, WB, IHC, FC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
RAB3B antibody; Ras related protein Rab 3B antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RAB3B recombinant protein (Accession Number: XM_017001958). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:114441(RAB3B antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:114441(RAB3B antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:114441(RAB3B antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human pancreas tissue slide using Catalog No:114441(RAB3B Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human pancreas tissue slide using Catalog No:114441(RAB3B Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:114441(RAB3B Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 HepG2 cells were stained with 0.2ug RAB3B antibody (Catalog No:114441, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
RAB3B represents RAB3B, member RAS oncogene family
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References
- Smith GA, Rocha EM, McLean JR. Progressive axonal transport and synaptic protein changes correlate with behavioral and neuropathological abnormalities in the heterozygous Q175 KI mouse model of Huntington's disease. Human molecular genetics. 23(17):4510-27. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"