Recombinant PUS1 protein (His tag)

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Recombinant Human Pseudouridylate Synthase 1 Homolog (PUS1) Fragment (aa 80-427) – a highly stable and soluble protein fragment ideal for structural studies and enzymatic assays. This 348-amino acid fragment retains key functional regions, enabling researchers to investigate pseudouridylation mechanisms in tRNA and mRNA modification with enhanced experimental versatility.

Pseudouridylate Synthase 1 (PUS1) is a critical enzyme in Homo sapiens that catalyzes pseudouridylation of both tRNAs and mRNAs, playing essential roles in RNA modification and regulation. It targets specific positions (27/28, 34, and 36) in tRNA anticodons and mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3'. Beyond RNA modification, PUS1 regulates pre-mRNA splicing and 3'-end processing through pseudouridylation near splice sites and influences nuclear receptor activity via modification of SRA1 mRNA. Subcellular localization varies by isoform: isoform 1 is mitochondrial, while isoform 2 is nuclear and cytoplasmic, highlighting its diverse functional contexts in cellular processes. This recombinant fragment (aa 80-427) is designed to support biochemical and structural analyses of these mechanisms.