• puroA peptide

puroA peptide

Not For Human Use, Lab Use Only.

Cat.#: 318786

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Optional Service: TFA RemovalWhat's this?

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Product Information

  • Product Name
    puroA peptide
  • Documents
  • Sequence Shortening
    H-FPVTWKWWKWWKG-NH2
  • Sequence
    H-Phe-Pro-Val-Thr-Trp-Lys-Trp-Trp-Lys-Trp-Trp-Lys-Gly-NH2
  • Length (aa)
    13
  • Peptide Purity (HPLC)
    95.2%
  • Molecular Formula
    C98H124N22O14
  • Molecular Weight
    1834.17
  • Source
    Synthetic
  • Form
    Powder
  • Description
    Puroindoline a, a wheat endosperm-specific protein containing a tryptophan-rich domain, was reported to have antimicrobial activities. A 13-residue fragment of puroindoline a (FPVTWRWWKWWKG-NH2) (puroA) exhibits activity against both gram-positive and gram-negative bacteria. PuroA interacted strongly with negatively charged phospholipid vesicles and induced efficient dye release from these vesicles, suggesting that the microbicidal effect of puroA may be due to interactions with bacterial membranes. puroA is located at the membrane interface, probably due to its high content of Trp residues that have a high propensity to partition into the membrane interface.
  • Storage Guidelines
    Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides
  • References
    • Arias M, Piga KB, Hyndman ME, Vogel HJ. Improving the Activity of Trp-Rich Antimicrobial Peptides by Arg/Lys Substitutions and Changing the Length of Cationic Residues. Biomolecules. 2018;8(2)
  • About TFA salt

    Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

    TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

    TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

    In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.

  • Molar Concentration Calculator

  • Dilution Calculator

  • Percent Concentration Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Peptide Property

  • Analysed Sequence:H-FPVTWKWWKWWKG-Amidation
  • Chemical Formula:C98H124N22O14
  • Sequence length:13
  • Extinction coefficient:28450 M-1cm-1
  • GRAVY:-0.92
  • Mw average:1834.17
  • Theoretical pI:10.84
  • Data Source:Peptide Property Calculator

GRAVY = grand average of hydropathy

X: Hydrophobic uncharged residues, like F I L M V W A and P

X: Basic residues, like R K H

X: Acidic residues, like D E

X: Polar uncharged residues, like G S T C N Q and Y

Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.

Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.

Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"