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Product Name
PRDX6 antibody
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Description
PRDX6 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in A549 cells, HeLa cells, SH-SY5Y cells. Positive IF detected in Hela cells. Positive IHC detected in human brain tissue. Observed molecular weight by Western-blot: 25-30kd
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
1 Cys antibody; 1 Cys peroxiredoxin antibody; 1 Cys PRX antibody; 24 kDa protein antibody; aiPLA2 antibody; Antioxidant protein 2 antibody; AOP2 antibody; KIAA0106 antibody; Liver 2D page spot 40 antibody; NSGPx antibody; p29 antibody; peroxiredoxin 6 antibody; PRDX6 antibody; PRX antibody; Red blood cells page spot 12 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PRDX6 recombinant protein (Accession Number: NM_004905). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:114178(PRDX6 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human brain using Catalog No:114178(PRDX6 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:114178(PRDX6 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using PRDX6 antibody Catalog No:114178 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-PRDX6 (IP:Catalog No:114178, 3ug; Detection:Catalog No:114178 1:500) with HeLa cells lysate 2800ug.
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Background
Prdx6(Peroxiredoxin-6) , a unique member of the peroxiredoxin family of antioxidants, is highly expressed in liver and protects cells from oxidative damage by reducing H2O2 and various lipid Peroxides(PMID: 17382207).It is also named as AOP2, KIAA0106. It can form a dimer(PMID:20500660).PRDX6 is expressed in all major organs, with a particularly high level in lung(PMID:15890616).
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References
- Aras S, Bai M, Lee I, Springett R, Hüttemann M, Grossman LI. MNRR1 (formerly CHCHD2) is a bi-organellar regulator of mitochondrial metabolism. Mitochondrion. 20:43-51. 2015.
- Ma S, Zhang X, Zheng L. Peroxiredoxin 6 Is a Crucial Factor in the Initial Step of Mitochondrial Clearance and Is Upstream of the PINK1-Parkin Pathway. Antioxidants & redox signaling. 2016.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"